LLPS000013:   LAF-1_m + LAF-1-mCherry-LAF-1_m
General information  
Link to protein
LAF-1    mCherry   
Componentsprotein(2)
Protein structureIDR + IDR-fold
Nucleic acid(s)-
Modification[LAF-1 RGG]2_Cleaved_Repeat(A tandem RGG protein in which two RGG domains are connected by a short linker)
[LAF-1 RGG]-[mCherry]-[LAF-1 RGG]_Fusion_Cleaved(A fusion protein construct in which two RGG domain of LAF-1 are separated with mCherry.)
Sequence length[LAF-1 RGG]2:340aa
[LAF-1 RGG]-[mCherry]-[LAF-1 RGG]:584aa(2-168;1-236;2-168)
Sequence>LLPS000013.1 synthetic protein construct [LAF-1 RGG]2|length=340aa
ESNQSNNGGSGNAALNRGGRYVPPHLRGGDGGAAAAASAGGDDRRGGAGGGGYRRGGGNS
GGGGGGGYDRGYNDNRDDRDNRGGSGGYGRDRNYEDRGYNGGGGGGGNRGYNNNRGGGGG
GYNRQDRGDGGSSNFSRGGYNNRDEGSDNRGSGRSYNNDRRDNGGDGEFGKLMESNQSNN
GGSGNAALNRGGRYVPPHLRGGDGGAAAAASAGGDDRRGGAGGGGYRRGGGNSGGGGGGG
YDRGYNDNRDDRDNRGGSGGYGRDRNYEDRGYNGGGGGGGNRGYNNNRGGGGGGYNRQDR
GDGGSSNFSRGGYNNRDEGSDNRGSGRSYNNDRRDNGGDG

>LLPS000013.2 A fusion protein construct [LAF-1 RGG]-[mCherry]-[LAF-1 RGG] with LAF-1 RGG domain fused to mCherry and LAF-1 RGG domain|length=584aa|residues=2-168
1-236
2-168
ESNQSNNGGSGNAALNRGGRYVPPHLRGGDGGAAAAASAGGDDRRGGAGGGGYRRGGGNS
GGGGGGGYDRGYNDNRDDRDNRGGSGGYGRDRNYEDRGYNGGGGGGGNRGYNNNRGGGGG
GYNRQDRGDGGSSNFSRGGYNNRDEGSDNRGSGRSYNNDRRDNGGDGGGGGSLEMVSKGE
EDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDIL
SPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYK
VKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTY
KAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKEFGGGGSESN
QSNNGGSGNAALNRGGRYVPPHLRGGDGGAAAAASAGGDDRRGGAGGGGYRRGGGNSGGG
GGGGYDRGYNDNRDDRDNRGGSGGYGRDRNYEDRGYNGGGGGGGNRGYNNNRGGGGGGYN
RQDRGDGGSSNFSRGGYNNRDEGSDNRGSGRSYNNDRRDNGGDG

Phase separation conditions  
Solute concentrationSalt concentrationBufferCrowding agentPressureTemperatureIncubation timeOthersDetection methodPhase separationMorphologyDescriptionReferenceIn vivo/In cell
6 µM [LAF-1 RGG]2;1 µM [LAF-1 RGG]-[mCherry]-[LAF-1 RGG]150 mM NaCl20 mM Tris, pH 7.5--25℃N/A-Microscopy Yes droplet refer to 30061688 Yes

Copyright@2019 University of Chinese Academy of Sciences.All Rights Reserved.

京ICP备19028953号-1