LLPS000020:   FUS
General information  
Link to protein
FUS   
Componentsprotein(1) + RNA
Protein structureIDR-fold
Nucleic acid(s)RNA_Blend(total Hela cellular RNA)
Modification[FUS]_WT
Sequence length[FUS]:526aa(1-526)
Sequence>LLPS000020 sp|P35637|FUS_HUMAN RNA-binding protein FUS OS=Homo sapiens OX=9606 GN=FUS PE=1 SV=1|length=526aa|residues=1-526
MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQ
SQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSS
SYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQD
QSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGYE
PRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIES
VADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFS
GNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQ
QRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYD
RGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY

Phase separation conditions  
Solute concentrationSalt concentrationBufferCrowding agentPressureTemperatureIncubation timeOthersDetection methodPhase separationMorphologyDescriptionReferenceIn vivo/In cell
5 µM [FUS];100-200 ng/ul RNA 75 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A-Microscopy Yes droplet refer to 29650702 Yes
5 µM [FUS];0-800 ng/ul rRNA75 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A-Microscopy Yes droplet refer to 29650702 Yes
5 µM [FUS];0-400 ng/ul tRNA75 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A-Microscopy Yes droplet refer to 29650702 Yes
5 µM [FUS];300 ng/ul RNA 75 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A-Microscopy No transparent solution refer to 29650702 Yes
5 µM [FUS];300 ng/ul RNA 75 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A5 ng/µl RNase AMicroscopy Yes droplet refer to 29650702 Yes
0.1-3 µM [FUS];50 ng/ul RNA 50-300 mM KCl;1 mM MgCl225 mM Tris/HCl buffer (pH 7.4), 1 mM TCEP--RTN/A-Microscopy Yes droplet refer to 29650702 Yes

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