LLPS000030:   FUS_m
General information  
Link to protein
FUS   
Componentsprotein(1)
Protein structureIDR
Nucleic acid(s)-
Modification[FUS PrD]_Cleaved_Mutation(64T65G to 64S)
Sequence length[FUS PrD]:173aa(1-174)
Sequence>LLPS000030 sp|P35637|FUS_HUMAN RNA-binding protein FUS OS=Homo sapiens OX=9606 GN=FUS PE=1 SV=1|length=173aa|residues=1-174|mutation=64T65G to 64S
MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQ
SQNSYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSS
YGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGG

Phase separation conditions  
Solute concentrationSalt concentrationBufferCrowding agentPressureTemperatureIncubation timeOthersDetection methodPhase separationMorphologyDescriptionReferenceIn vivo/In cell
100 µM [FUS PrD]150 mM NaCl25 mM Tris pH 7.5--22℃after 30-45 min-Microscopy No transparent solution refer to 30791483 N/A
100 µM [FUS PrD]150 mM NaCl25 mM Tris pH 7.5--4℃after 30-45 min-Microscopy(reversible) Yes liquid droplet refer to 30791483 N/A
30-175 µM [FUS PrD]150 mM NaCl25 mM Tris pH 7.50-100 mg/ml PEG8000-22℃after 2 min-Turbidity measurement;Microscopy Phase diagram droplet refer to 30791483 N/A
10 µM [FUS PrD]150 mM NaCl25 mM Tris pH 7.525-100 mg/ml PEG8000-22℃after 30-45 min-Microscopy;FRAP Yes liquid droplet refer to 30791483 N/A

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