LLPS000075: FUS_m

General information

Link to protein

FUS

Components	protein(1) + RNA
Protein structure	IDR-fold
Nucleic acid(s)	RNA_Blend(Torula yeast RNA)
Modification	[FUS]_Mutation(series of S or T at 26, 30, 68, 84, 87, 117 are mutated to E)
Sequence length	[FUS]:526aa(1-526)
Sequence	>LLPS000075 sp\|P35637\|FUS_HUMAN RNA-binding protein FUS OS=Homo sapiens OX=9606 GN=FUS PE=1 SV=1\|length=526aa\|residues=1-526\|mutation=S or T26:30:68:84:87:117E MASNDYTQQATQSYGAYPTQPGQGYEQQSEQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQ SQNTGYGEQSTPQGYGSTGGYGSEQSEQSSYGQQSSYPGYGQQPAPSSTSGSYGSSEQSS SYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQD QSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGYE PRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIES VADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFS GNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQ QRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYD RGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY

Phase separation conditions

Solute concentration	Salt concentration	Buffer	Crowding agent	Pressure	Temperature	Incubation time	Others	Detection method	Phase separation	Morphology	Description	Reference	In vivo/In cell
5 µM [FUS];0.1 mg/mL RNA	150 mM NaCl	20 mM Tris pH 7.4	-	-	25℃	within 1 d	agitation 1200 rpm	Microscopy	Yes	droplet	refer to	28790177	Yes
5 µM [FUS];0.05-0.5 mg/mL RNA	150 mM NaCl	20 mM Tris pH 7.4	-	-	25℃	after 45 min	-	Turbidity measurement	Yes	droplet	refer to	28790177	Yes

Copyright@2019 University of Chinese Academy of Sciences.All Rights Reserved.

京ICP备19028953号-1