LAF-1 D0PV95
Full protein nameATP-dependent RNA helicase laf-1;DEAD-box RNA helicase laf-1
Gene namelaf-1;Y71H2AM.19
SpeciesCaenorhabditis elegans
LocalizationCytoplasm; Cytoplasmic granule.
DescriptionLAF-1, an ATP-dependent DDX3 RNA helicase found in P granules, plays a role in RNA remodeling, but is not required for RNA unwinding. Binds to RNA in a concentration-dependent manner to stimulate annealing between two complementary strands of RNA. This process is also dependent upon ATP; ATP reduces binding to RNA and subsequently diminishes RNA annealing. Promotes liquid-liquid phase separation of P granules, which is a process important for intracellular organization and stress granule assembly. The purely viscous properties highly tunable by salt and RNA concentration. RNA decreases viscosity and increases molecular dynamics within the droplet.
LAF-1 is comprised of an intrinsically disordered RGG domain at its N terminus, followed by a helicase domain and disordered C-terminal domain. The N-terminal, arginine/glycine rich, intrinsically disordered protein (IDP) domain of LAF-1 is necessary and sufficient for both phase separation and RNA-protein interactions. The C-terminal of LAF-1 (LAF-1^C) contains a R/G/Q rich domain,which doesn’t undergo phase transitions along, while the WT LAF-1 and LAF-1^RGG does.
GO termATP binding
helicase activity
protein homodimerization activity
RNA strand annealing activity
RNA-dependent ATPase activity
Full sequence length708
Database linked
MobiDB : D0PV95   
IDR Region
    1 708
LCR Region
    1 708

12
Entry IDProtein(s)Protein typeFusionCleavedRepeatMutationPTMSequence lengthProtein structure typeMain componentsNucleic acid
LLPS000001LAF-1N-----LAF-1:708aa(1-708)IDR-foldprotein(1)-
LLPS000002LAF-1N-----LAF-1:708aa(1-708)IDR-foldprotein(1) + RNApolyA:15nt
LLPS000003LAF-1N-----LAF-1:708aa(1-708)IDR-foldprotein(1) + RNApolyA:3000nt
LLPS000004LAF-1N-----LAF-1:708aa(1-708)IDR-foldprotein(1) + RNApolyA:30nt
LLPS000005LAF-1N-----LAF-1:708aa(1-708)IDR-foldprotein(1) + RNApolyU:50nt
LLPS000006LAF-1_mN-(RGG)---LAF-1_m:167aa(2-168)IDRprotein(1)-
LLPS000007LAF-1_mN-(RGG)---LAF-1_m:168aa(1-168)IDRprotein(1)-
LLPS000008LAF-1_mN-(RGG)2--LAF-1_m:340aaIDRprotein(1)-
LLPS000009LAF-1_mN-(RGG)3--LAF-1_m:539aaIDRprotein(1)-
LLPS000010LAF-1_mN-(△C)---LAF-1_m:647aa(1-647)IDR-foldprotein(1)-
LLPS000011LAF-1_mN-(△RGG)---LAF-1_m:541aa(168-708)IDR-foldprotein(1)-
LLPS000012LAF-1_m
EGFP
N
N
-
-
(RGG)
-
2
-
-
-
-
-
LAF-1_m:340aa
EGFP:239aa(1-239)
IDR
fold
protein(2)-
LLPS000013LAF-1_m
LAF-1-mCherry-LAF-1_m
N
N
-
Yes
(RGG)
(RGG),-,(RGG)
2
-
-
-
-
-
LAF-1_m:340aa
LAF-1-mCherry-LAF-1_m:584aa(2-168;1-236;2-168)
IDR
IDR-fold
protein(2)-
LLPS000014LAF-1_m
mCherry
N
N
-
-
(RGG)
-
2
-
-
-
-
-
LAF-1_m:340aa
mCherry:236aa(1-236)
IDR
fold
protein(2)-
LLPS000015LAF-1_m
mCherry-LAF-1_m
N
N
-
Yes
(RGG)
-,(RGG)
2
-
-
-
-
-
LAF-1_m:340aa
mCherry-LAF-1_m:419aa(1-236;2-168)
IDR
IDR-fold
protein(2)-
LLPS001027SZ1-LAF-1_m
EGFP-SZ2
mCherry-SZ2
D,N
N,D
N,D
Yes
Yes
Yes
-,(RGG)
-
-
-,2
-
-
-
-
-
-
-
-
SZ1-LAF-1_m:392aa(47;340)
EGFP-SZ2:299aa(1-239;50)
mCherry-SZ2:296aa(1-236;50)
IDR
IDR-fold
IDR-fold
protein(3)-
12